Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Cofilin (D59) Antibody #3318

Applications Reactivity MW (kDa) Source
W H M R Mk B 19 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cofilin (D59) Antibody detects endogenous levels of total cofilin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asp59 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Cofilin (D59) Antibody.

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

  1. Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
  2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
  3. Arber, S. et al. (1998) Nature 393, 805-809.
  4. Yang, N. et al. (1998) Nature 393, 809-812.
  5. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
  6. Nebl, G. et al. (1996) J. Biol. Chem. 271, 26276-26280.

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