Product Pathways - Lymphocyte Signaling
MNDA (3C1) Rat mAb #3329
|3329S||100 µl (10 western blots)||---||In Stock||---|
|3329||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
MNDA (3C1) Rat mAb detects endogenous levels of total MNDA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with nuclear extract from human granulocytes.
Western blot analysis of extracts from HL-60 and THP-1 cells using MNDA (3C1) Rat mAb.
Flow cytometric analysis of Jurkat cells (red) and THP-1 cells (blue) using MNDA (3C1) Rat mAb.
Confocal immunofluorescent analysis of THP-1 cells (left) and Jurkat cells (right) using MNDA (3C1) Rat mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
MNDA (myeloid cell nuclear differentiation antigen) is a transcription factor constitutively expressed in peripheral blood granulocytes and monocytes; low expression is also found in a subset of B cells (1,2). MNDA is a member of the interferon (IFN)-regulated 200 family of genes, which contain one or more copies of a partially conserved domain of 200 amino acid residues thought to mediate protein-protein interaction (3). MNDA may play a role in apoptosis and its expression is reduced in myelodysplastic syndromes (MDS) (4). MNDA has been proposed to be a marker for nodal marginal zone lymphoma (5).
- McClintock-Treep, S.A. et al. (2011) Am J Clin Pathol 135, 380-5. Applications: Flow Cytometry.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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