Cell Signaling Technology

Product Pathways - Phosphatases

PTPA/PPP2R4 Antibody #3330

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PTPA/PPP2R4 Antibody detects endogenous levels of total PTPA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PTPA. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PTPA/PPP2R4 Antibody.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). Active protein phosphatase 2A is composed of both structural (A) and catalytic (C) proteins, and its activity relies on interaction with regulatory (B) subunits. An important PP2A regulatory subunit is PP2A phosphatase activator (PTPA), also known as the PP2A activator regulatory subunit 4 (PPP2R4) (4). This PTPA regulatory protein binds ATP and has isomerase (PPIase) activity, suggesting that PP2A regulation involves a change in phosphatase conformation. The addition of ATP (and Mg2+) results in a correlated increase in both PP2A activation and PTPA isomerase activity (5). While the exact mechanism is still under consideration, evidence suggests that binding of PTPA to the PP2A A-C dimer produces a conformational change in PP2A that shifts phosphatase substrate specificity from phosphoserine to phosphotyrosine substrates (6).

  1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39.
  2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35.
  3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91.
  4. Fellner, T. et al. (2003) Genes Dev 17, 2138-50.
  5. Jordens, J. et al. (2006) J Biol Chem 281, 6349-57.
  6. Chao, Y. et al. (2006) Mol Cell 23, 535-46.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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