Product Pathways - Jak/Stat Pathway
Phospho-Jak1 (Tyr1022/1023) Antibody #3331
|W||H M (R) (B)||Endogenous||130||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Jak1 (Tyr1022/1023) Antibody detects endogenous levels of Jak1 only when phosphorylated at tyrosines 1022/1023. This antibody may cross-react with phospho-Jak2.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1022/1023 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography.
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).(This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.)
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
- Duan, Z. et al. (2006) Clin Cancer Res 12, 6844-52. Applications: Western Blotting
- Okumura, M. et al. (2011) Biochem Pharmacol , . Applications: Western Blotting
- Zhang, W.N. et al. (2012) J Biol Chem 287, 382-92. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.