Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Jak1 (Tyr1022/1023) Antibody #3331

Applications Reactivity MW (kDa) Source
W H M (R) (B) 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Jak1 (Tyr1022/1023) Antibody detects endogenous levels of Jak1 only when phosphorylated at tyrosines 1022/1023. This antibody may cross-react with phospho-Jak2.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1022/1023 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells treated with IL-4 (100 ng/ml) for the indicated times, using Phospho-Jak1 (Tyr1022/1023) Antibody (upper) or Jak1 Antibody (lower).

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).(This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.)

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-1635.
  3. VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
  4. Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
  5. Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
  6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

Application References

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