Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Jak1 Antibody #3332

Applications Reactivity MW (kDa) Source
W IP H M (R) (B) 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Jak1 Antibody detects endogenous levels of total Jak1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr1022/1023 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 and BaF3 cells, using Jak1 Antibody.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-1635.
  3. VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
  4. Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
  5. Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.

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