Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

ALK (C26G7) Rabbit mAb #3333

Applications Reactivity MW (kDa) Source Isotype
W H 80 kDa (NPM-ALK), 200 kDa (ALK) Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

ALK (C26G7) Rabbit mAb detects endogenous levels of total ALK protein. This antibody does not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a recombinant GST-fusion protein containing amino acids 1405-1537 of human ALK.

Western Blotting

Western Blotting

Western blot analysis of extracts from Karpas-299 and Sup-M2 cells using ALK (C26G7) Rabbit mAb.

Background

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas. (5).

  1. Stoica, G.E. et al. (2001) J. Biol. Chem. 276, 16772-16779.
  2. Iwahara, T. et al. (1997) Oncogene 14, 439-449.
  3. Morris, S.W. et al. (1997) Oncogene 14, 2175-2188.
  4. Morris, S.W. et al. (1994) Science 263, 1281-1284.
  5. Bai, R.Y. et al. (1998) Mol. Cell Biol. 18, 6951-6961.

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