Product Pathways - MAPK Signaling
Ras (27H5) Rabbit mAb #3339
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk Dm | Endogenous | 21 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Ras (27H5) Rabbit mAb detects endogenous levels of total K-Ras, H-Ras and N-Ras proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the amino terminus of human K-Ras.
Western Blotting
Western blot analysis of extracts from 293T, C2C12 and C6 cells using Ras (27H5) Rabbit mAb.
Background
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654.
- Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283.
- Buday, L. and Downward, J. (1993) Cell 73, 611-620.
- Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431.
- Bos, J.L. (1989) Cancer Res. 49, 4682-4689.
- Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
