Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Phospho-ALK (Tyr1586) Antibody #3343

Applications Reactivity MW (kDa) Source
W H 80 NPM-ALK. 200 ALK. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-ALK (Tyr1586) Antibody detects ALK only when phosphorylated at tyrosine 1586 (equivalent to Tyr646 of NPM-ALK).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1586 of human ALK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Sup-M2 cells, using Phospho-ALK (Tyr1586) Antibody (A,B) or ALK Antibody (C,D). The phospho-specificity of the antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B,D) after Western transfer.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells expressing different activated tyrosine kinases, using Phospho-ALK (Tyr1586) Antibody (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-ALK (Tyr1586) Antibody shows no cross-reactivity with other tyrosine-phosphorylated tyrosine kinases.

Background

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas. (5).

  1. Stoica, G.E. et al. (2001) J. Biol. Chem. 276, 16772-16779.
  2. Iwahara, T. et al. (1997) Oncogene 14, 439-449.
  3. Morris, S.W. et al. (1997) Oncogene 14, 2175-2188.
  4. Morris, S.W. et al. (1994) Science 263, 1281-1284.
  5. Bai, R.Y. et al. (1998) Mol. Cell Biol. 18, 6951-6961.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes