Product Pathways - Jak/Stat Pathway
Jak1 (6G4) Rabbit mAb #3344
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R (Mk) (Pg) (Dg) | Endogenous | 130 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Dg=Dog
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Jak1 (6G4) Rabbit mAb detects endogenous levels of total Jak1 protein. It does not cross react with other Jak family members.
Source / Purification
Rabbit monoclonal antibodies are prepared from rabbit spleens immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Cys775 of Jak1.
Western Blotting
Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines, using Jak1 (6G4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344 in the presence of control peptide (left) or Jak1 Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Jak1 (6G4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Jak1 (6G4) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Hela cells, using Jak1 (6G4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
