Product Pathways - MAPK Signaling
SEK1 (5C10) Rabbit mAb #3346
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | 44 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
SEK1 (5C10) Rabbit mAb detects endogenous levels of total SEK1 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of human SEK1.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
- Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
- Sanchez, I. et al. (1994) Nature 372, 794-798.
- Yan, M. et al. (1994) Nature 372, 798-800.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
- Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.