Cell Signaling Technology

Product Pathways - MAPK Signaling

SEK1 (5C10) Rabbit mAb #3346

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (Dg) Endogenous 44 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SEK1 (5C10) Rabbit mAb detects endogenous levels of total SEK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human SEK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and COS cells, using SEK1 (5C10) Rabbit mAb.

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

  1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
  2. Sanchez, I. et al. (1994) Nature 372, 794-798.
  3. Yan, M. et al. (1994) Nature 372, 798-800.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
  5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products