Cell Signaling Technology

Product Pathways - Neuroscience

SYT1 Antibody #3347

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F H M R Mk Endogenous 60 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SYT1 Antibody detects endogenous levels of total SYT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SYT1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SYT1 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of C2C12 cells using SYT1 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using SYT1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+ (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6).

  1. Fukuda, M. and Mikoshiba, K. (2001) Biochem Biophys Res Commun 281, 1226-33.
  2. Südhof, T.C. (2002) J Biol Chem 277, 7629-32.
  3. Fernández-Chacón, R. et al. (2001) Nature 410, 41-9.
  4. Lynch, K.L. et al. (2007) Mol Biol Cell 18, 4957-68.
  5. Gauthier, B.R. and Wollheim, C.B. (2008) Am J Physiol Endocrinol Metab 295, E1279-86.
  6. Bai, J. et al. (2004) Neuron 41, 929-42.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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