Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H | 80 kDa NPM-ALK fusion; 200 kDa ALK | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-ALK (Tyr1586) (3B4) Rabbit mAb detects ALK only when phosphorylated at Tyr1586 (equivalent to Tyr646 of NPM-ALK). This antibody may cross-react with other activated protein tyrosine kinases including EGFR.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Tyr1586 of human ALK.
Western Blotting
Western blot analysis of Sup-M2 cell lysate, using Phospho-ALK (Tyr1586) (3B4) Rabbit mAb (A and B), and ALK Antibody (C and D).The phospho-specificity of this antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B and D) after Western transfer.
Background
Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas. (5).
- Stoica, G.E. et al. (2001) J. Biol. Chem. 276, 16772-16779.
- Iwahara, T. et al. (1997) Oncogene 14, 439-449.
- Morris, S.W. et al. (1997) Oncogene 14, 2175-2188.
- Morris, S.W. et al. (1994) Science 263, 1281-1284.
- Bai, R.Y. et al. (1998) Mol. Cell Biol. 18, 6951-6961.
Application References
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Companion Products
- 3343 Phospho-ALK (Tyr1586) Antibody
- 3341 Phospho-ALK (Tyr1604) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3333 ALK (C26G7) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
