Cell Signaling Technology

Product Pathways - Protein Folding/Stability

SGTA Antibody #3349

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 34 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

SGTA Antibody detects endogenous levels of total SGTA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SGTA. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from H1703 cells transfected with non-targeted (-) or SGTA targeted (+) siRNA. SGTA Antibody confirms the silencing of protein expression while β-Actin Antibody #4967 is used to control for loading.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SGTA Antibody.

Background

SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6).

  1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
  2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
  3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
  6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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