Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

ApoA1 (5F4) Mouse mAb #3350

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 25 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ApoA1 (5F4) Mouse mAb detects endogenous level of total ApoA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a full-length recombinant human ApoA1.

Western Blotting

Western Blotting

Western blot analysis of decreasing concentrations of ApoA1 protein purified from human serum using ApoA1 (5F4) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 cells using ApoA1 (5F4) Mouse mAb.

Background

ApoAI (Apolipoprotein A1) is a major component of high density lipoprotein (HDL, the “good cholesterol”) in plasma. It is produced in the liver and small intestine. ApoA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) that is responsible for the formation of plasma cholesteryl esters and promotes cholesterol efflux from tissues to the liver for excretion. Defects in ApoA1 are associated with high density lipoprotein deficiency (HDLD) and systemic non-neuropathic amyloidosis (1-3).

  1. Calabresi, L. and Franceschini, G. (1997) Curr Opin Lipidol 8, 219-24.
  2. Miller, M. and Zhan, M. (2004) Curr Opin Cardiol 19, 380-4.
  3. Sviridov, D. and Nestel, P.J. (2007) Curr Opin Lipidol 18, 157-63.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products