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Stathmin Antibody #3352

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P	H M R Mk	Endogenous	19	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3352:

IHC / Paraffin, Western Blotting

Specificity / Sensitivity

Stathmin Antibody detects endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins such as SCG10, SCLIP and RB3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser38 of human stathmin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).

1. Marklund, U. et al. (1994) Eur. J. Biochem. 225, 53-60.
2. le Gouvello, S. et al. (1998) J. Immunol. 161, 1113-1133.
3. Mistry, S.J. and Atweh, G.F. (2001) J. Biol. Chem. 276, 31209-31215.
4. Gavet, O. et al. (1998) J. Cell Sci. 111, 3333-3346.
5. Luo, X. N. et al. (1994) J. Biol. Chem. 269, 10312-10318.
6. Vancompernolle, K. et al. (2000) J. Biol. Chem. 275, 33876-33882.
7. Daub, H. et al. (2001) J. Biol. Chem. 276, 1677-1680.
8. Di Paolo, G. et al. (1996) J. Cell. Biol. 133, 1383-1390.

Application References

• Saal, L.H. et al. (2007) Proc Natl Acad Sci U S A 104, 7564-9. Applications: Western Blotting
• Liu, Z. et al. (2005) Cancer Res 65, 1647-54. Applications: Western Blotting
• Verma, N.K. et al. (2009) J Biol Chem 284, 12349-62. Applications: Western Blotting
• Salvesen, H.B. et al. (2009) Proc Natl Acad Sci U S A 106, 4834-9. Applications: IHC-P (paraffin)
• Li, F. et al. (2011) PLoS One 6, e21977. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 8112 SignalStain^® Antibody Diluent
• 8059 SignalStain^® DAB Substrate Kit
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

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For Research Use Only. Not For Use In Diagnostic Procedures.