Cell Signaling Technology

Product Pathways - Development

CACYBP Antibody #3354

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P F H M R Mk Endogenous 26 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CACYBP Antibody detects endogenous levels of total CACYBP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp162 of human CACYBP. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CACYBP Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CACYBP Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using CACYBP Antibody (blue) compared to a nonspecific negative control antibody (red).


Background

Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9).

  1. Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
  2. Fukushima, T. et al. (2006) Immunity 24, 29-39.
  3. Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
  4. Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
  5. Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
  6. Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
  7. Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
  8. Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
  9. Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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