Product Pathways - Neuroscience
Phospho-CaMKII (Tyr231) Antibody #3356
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R (H) | Endogenous | 50 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3356:
- Western Blotting
Specificity / Sensitivity
Phospho-CaMKII (Tyr231) Antibody detects endogenous levels of CaMKII only when phosphorylated at Tyr231.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr231 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-CaMKII (Tyr231) Antibody (upper) or CaMKII Antibody (lower).
Background
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).
Phospho-CaMKII (Tyr231) Antibody is directed against a previously unpublished CaMKII tyrosine phosphorylation site at Tyr231 that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of CamKII at Tyr231 was observed in extracts isolated from ischemic rat brain.
- Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008-36013.
- Barria, A. et al. (1997) Science 276, 2042-2045.
- Barkai, U. et al. (2000) Mol. Endocrinol. 14, 554-563.
- Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
Application References
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Companion Products
- 3361 Phospho-CaMKII (Thr286) Antibody
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- 3362 CaMKII (pan) Antibody
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- 2482 PLCbeta3 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
