Product Pathways - PI3K / Akt Signaling
PI3 Kinase Class III (D4E2) Rabbit mAb #3358
PhosphoSitePlus® protein, site, and accession data: PIK3C3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 100 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3358:
- Western Blotting
Specificity / Sensitivity
PI3 Kinase Class III (D4E2) Rabbit mAb detects endogenous levels of total PI3K class III protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the amino-terminal sequence of human PI3K class III.
Western Blotting
Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb.
Background
Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).
- Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35.
- Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85.
- Kihara, A. et al. (2001) J Cell Biol 152, 519-30.
- Corvera, S. (2001) Traffic 2, 859-66.
- Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
Application References
- Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting
- Yoshioka, K. et al. (2012) Nat Med 18, 1560-9. Applications: Western Blotting
- Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
