Product Pathways - Neuroscience
Phospho-CaMKII (Thr286) Antibody #3361
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 50 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-CaMKII (Thr286) Antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 286.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding threonine 286 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Neuro2A cells treated with calcium ionophore (A23187, 2 µM) after a 20-minute preincubation with forskolin (30 μM), using Phospho-CaMKII (Thr286) Antibody (upper) or CaMKII Antibody #3362 (lower).
Western Blotting
Western blot analysis of extracts from Neuro2A cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CaMKII (Thr286) Antibody (upper) or CaMKII Antibody #3362 (lower).
Background
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).
- Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008-36013.
- Barria, A. et al. (1997) Science 276, 2042-2045.
- Means, A.R. (2000) Mol. Endocrinol. 14, 4-12.
- Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
- J Neurochem. 2008 Mar;104(5):1372-86. Epub 2007 Nov 14.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
