Product Pathways - Neuroscience
CaMKII (pan) Antibody #3362
PhosphoSitePlus® protein, site, and accession data: CaMK2-alpha, CaMK2-beta, CaMK2-delta, CaMK2-gamma
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 60, 50 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3362:
- Western Blotting
Specificity / Sensitivity
CaMKII (pan) Antibody detects endogenous levels of total CaMKII.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal region of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from rat and mouse brain using CaMKII (pan) Antibody.
Background
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).
- Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008-36013.
- Barria, A. et al. (1997) Science 276, 2042-2045.
- Barkai, U. et al. (2000) Mol. Endocrinol. 14, 554-563.
- Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
Application References
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Companion Products
- 3361 Phospho-CaMKII (Thr286) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4436 CaMKII (pan) (D11A10) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
