Product Pathways - Neuroscience
CaMKII pan Antibody #3362
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IF-IC | H M R Mk | 50 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CaMKII Antibody detects endogenous levels of total CaMKII beta. This antibody may also detect the alpha, delta, and gamma subunits of CaMKII but preferentially recognizes CaMKII beta isoforms 1-7.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the amino-terminal region of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).
- Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008-36013.
- Barria, A. et al. (1997) Science 276, 2042-2045.
- Means, A.R. (2000) Mol. Endocrinol. 14, 4-12.
- Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
Application References
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