Cell Signaling Technology

Product Pathways - Neuroscience

CaMKII pan Antibody #3362

Applications Reactivity MW (kDa) Source
W IF-IC H M R Mk 50 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CaMKII Antibody detects endogenous levels of total CaMKII beta. This antibody may also detect the alpha, delta, and gamma subunits of CaMKII but preferentially recognizes CaMKII beta isoforms 1-7.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the amino-terminal region of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human (SH-SY5Y and SK-N-MC, lanes 1 and 2), mouse (Neuro2A and L cell, lanes 3 and 4), rat (PC12 and C6, lanes 5 and 6) and monkey (COS, lane 7) cell lines using CaMKII Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells using CaMKII Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 (red).

Background

CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).

  1. Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008-36013.
  2. Barria, A. et al. (1997) Science 276, 2042-2045.
  3. Means, A.R. (2000) Mol. Endocrinol. 14, 4-12.
  4. Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.

Application References

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