Product Pathways - Translational Control
Dicer Antibody #3363
PhosphoSitePlus® protein, site, and accession data: Dicer1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 220 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Dicer Antibody detects endogenous levels of total Dicer protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Dicer. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Dicer Antibody.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Dicer siRNA I #6576 (+) or SignalSilence® Dicer siRNA II (+), using Dicer Antibody #3363 and α-Tubulin (11H10) Rabbit mAb #2125. The dicer antibody confirms silencing of dicer expression, while α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of dicer siRNA.
Background
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3).
- Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60.
- Lee, Y. et al. (2003) Nature 425, 415-9.
- Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108.
Application References
- Smith, J.L. et al. (2012) J Virol 86, 5278-87. Applications: Western Blotting
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- 2071 Mili Antibody
- 6576 SignalSilence® Dicer siRNA I
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
