Product Pathways - Translational Control
Dicer Antibody #3363
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Dicer Antibody detects endogenous levels of total Dicer protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Dicer. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Dicer siRNA I #6576 (+) or SignalSilence® Dicer siRNA II (+), using Dicer Antibody #3363 and α-Tubulin (11H10) Rabbit mAb #2125. The dicer antibody confirms silencing of dicer expression, while α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of dicer siRNA.
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3).
- Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60.
- Lee, Y. et al. (2003) Nature 425, 415-9.
- Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108.
- Smith, J.L. et al. (2012) J Virol 86, 5278-87. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.