Product Pathways - Translational Control
Drosha (D28B1) Rabbit mAb #3364
|W IP||H M||Endogenous||160||Rabbit IgG|
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Drosha (D28B1) Rabbit mAb detects endogenous levels of total Drosha protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly953 of human Drosha.
Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8 (2). Drosha, along with DGCR8, is necessary for miRNA biogenesis (3).
- Lee, Y. et al. (2003) Nature 425, 415-9.
- Denli, A.M. et al. (2004) Nature 432, 231-5.
- Gregory, R.I. et al. (2004) Nature 432, 235-40.
- Pickering, B.F. et al. (2011) J Biol Chem 286, 44095-103. Applications: Western Blotting
- Smith, J.L. et al. (2012) J Virol 86, 5278-87. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.