Cell Signaling Technology

Product Pathways - Translational Control

Drosha (D28B1) Rabbit mAb #3364

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 160 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Drosha (D28B1) Rabbit mAb detects endogenous levels of total Drosha protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly953 of human Drosha.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Drosha (D28B1) Rabbit mAb.

Background

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8 (2). Drosha, along with DGCR8, is necessary for miRNA biogenesis (3).

  1. Lee, Y. et al. (2003) Nature 425, 415-9.
  2. Denli, A.M. et al. (2004) Nature 432, 231-5.
  3. Gregory, R.I. et al. (2004) Nature 432, 235-40.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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