Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

Neuropilin-2 (D39A5) XP® Rabbit mAb #3366

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-F M R Endogenous 130 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Neuropilin-2 (D39A5) XP® Rabbit mAb detects endogenous levels of total neuropilin-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Neuropilin-2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain and NIH/3T3 cells using Neuropilin-2 (D39A5) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse pancreas using Neuropilin-2 (D39A5) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of E14.5 mouse embryo using Neuropilin-2 (D39A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-F

IF-F

Confocal immunofluorescent analysis of rat pancreas using Neuropilin2 (D39A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

Neuropilin-2, a single pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g. pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5).

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
  3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
  4. Favier, B. et al. (2006) Blood 108, 1243-50.
  5. Chen, C. et al. (2005) World J Surg 29, 271-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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