Product Pathways - Neuroscience
Neuropilin-2 (D39A5) XP® Rabbit mAb #3366
|3366S||100 µl (10 western blots)||---||In Stock||---|
|3366P||40 µl (4 western blots)||---||In Stock||---|
|3366||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Mouse, Rat||Endogenous||130||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Neuropilin-2 (D39A5) XP® Rabbit mAb detects endogenous levels of total neuropilin-2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Neuropilin-2 protein.
Western blot analysis of extracts from rat brain and NIH/3T3 cells using Neuropilin-2 (D39A5) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Neuropilin-2 (D39A5) XP® Rabbit mAb.
Confocal immunofluorescent analysis of E14.5 mouse embryo using Neuropilin-2 (D39A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).
Neuropilin-2, a single pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g. pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.