Product Pathways - Companion Products
GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) #3368
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | All | Transfected Only | Mouse IgG2a |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 3368:
- Flow, Immunofluorescence
Specificity / Sensitivity
GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio 2-6.
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with GST (green), using GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate.)
IF-IC
Confocal immunofluorescent analysis of COS cells, mock transfected (left) or transfected with a GST-tagged protein (right), using GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with GST-tagged protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2624 GST (26H1) Mouse mAb
- 3445 GST (26H1) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 2622 GST Antibody
- 2372 β-Gal (14B7) Mouse mAb
- 2367 HA-Tag (6E2) Mouse mAb
- 2350 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2365 His-Tag Antibody
- 2396 MBP (8G1) Mouse mAb
- 2272 Myc-Tag Antibody
- 2276 Myc-Tag (9B11) Mouse mAb
- 2279 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2233 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 3444 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 647 Conjugate)
The Alexa Fluor® dye antibody conjugates in this product are sold under license for Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
