Product Pathways - MAPK Signaling
Erk5 Antibody #3372
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 115 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Erk5 Antibody detects endogenous levels of total Erk5 protein. This antibody does not cross-react with Erk1 or Erk2.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human Erk5. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Erk5, also known as BMK1 (big mitogen-activated kinase 1), contains a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the amino-terminal kinase domain with Erk1/2. However, its long carboxy-terminal domain makes Erk5 a unique MAP kinase. Erk5 is activated by a wide variety of extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxidative stress (1,2). The kinase directly upstream of Erk5 has been identified as MEK5 (1). MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3-5). Growing evidence indicates that the Erk5 pathway is involved in proliferation, differentiation, oncogenesis and neuronal development (1,6-8).
- Kato, Y. et al. (1997) EMBO J. 16, 7054-7066.
- Fukuhara, S. et al. (2000) J. Biol. Chem. 275, 21730-21736.
- Chao, T. et al. (1999) J. Biol. Chem. 274, 36035-36038.
- Sun, W. et al. (2001) J. Biol. Chem. 276, 5093-5100.
- Chiariello, M. et al. (2000) Mol. Cell. Biol. 20, 1747-1758.
- Dinev, D. et al. (2001) EMBO Rep. 2, 829-834.
- Esparis-Ogando, A. et al. (2002) Mol. Cell. Biol. 22, 270-285.
- Watson, F. L. et al. (2001) Nat. Neurosci. 4, 981-988.
Application References
- Mulloy, R. et al. (2003) Activation of cyclin D1 expression by the Erk5 cascade. Oncogene 22, 5387-5398. This article references the use of Erk5 Antibody in the following applications: Western Blotting
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Companion Products
- 3371 Phospho-Erk5 (Thr218/Tyr220) Antibody
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.