Cell Signaling Technology

Product Pathways - MAPK Signaling

Erk5 Antibody #3372

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 115 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Erk5 Antibody detects endogenous levels of total Erk5 protein. This antibody does not cross-react with Erk1 or Erk2.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human Erk5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or sorbitol-treated and NIH/3T3 cells, untreated or PDGF-treated, using Erk5 Antibody.

Background

Erk5, also known as BMK1 (big mitogen-activated kinase 1), contains a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the amino-terminal kinase domain with Erk1/2. However, its long carboxy-terminal domain makes Erk5 a unique MAP kinase. Erk5 is activated by a wide variety of extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxidative stress (1,2). The kinase directly upstream of Erk5 has been identified as MEK5 (1). MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3-5). Growing evidence indicates that the Erk5 pathway is involved in proliferation, differentiation, oncogenesis and neuronal development (1,6-8).

  1. Kato, Y. et al. (1997) EMBO J. 16, 7054-7066.
  2. Fukuhara, S. et al. (2000) J. Biol. Chem. 275, 21730-21736.
  3. Chao, T. et al. (1999) J. Biol. Chem. 274, 36035-36038.
  4. Sun, W. et al. (2001) J. Biol. Chem. 276, 5093-5100.
  5. Chiariello, M. et al. (2000) Mol. Cell. Biol. 20, 1747-1758.
  6. Dinev, D. et al. (2001) EMBO Rep. 2, 829-834.
  7. Esparis-Ogando, A. et al. (2002) Mol. Cell. Biol. 22, 270-285.
  8. Watson, F. L. et al. (2001) Nat. Neurosci. 4, 981-988.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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