Cell Signaling Technology

Product Pathways - Neuroscience

TrkC (C44H5) Rabbit mAb #3376

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Endogenous 145, 100 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TrkC (C44H5) Rabbit mAb detects endogenous levels of total TrkC protein. The antibody does not cross-react with TrkA or TrkB.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gly50 of human TrkC.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using TrkC (C44H5) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, wild-type (upper left), NIH/3T3/TrkA (upper right), NIH/3T3/TrkB (lower left) or NIH/3T3/TrkC (lower right) using TrkC (C44H5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).

The phosphorylation sites are conserved between TrkA and TrkC: Tyr490 of TrkA corresponds to Tyr516 in TrkC, and Tyr674/675 of TrkA to Tyr709/710 in TrkC of the human sequence (7). Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma (8).

  1. Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
  2. Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
  3. Stephens, R.M. et al. (1994) Neuron 12, 691-705.
  4. Obermeier, A. et al. (1993) EMBO J 12, 933-41.
  5. Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
  6. Yao, R. and Cooper, G.M. (1995) Science 267, 2003-6.
  7. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
  8. Segal, R.A. et al. (1994) Proc Natl Acad Sci U S A 91, 12867-71.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products