Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-NMDAR1 (Ser890) Antibody #3381

Applications Reactivity MW (kDa) Source
W IF-F H M R 120 Rabbit

Applications Key:  W=Western Blotting  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-NMDAR1 (Ser890) Antibody detects transfected NMDAR1 only when phosphorylated at serine 890.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding serine 890 of human NMDAR1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from parental (COS) or NMDAR1-transfected (COS/NMDAR1) cells, untreated or TPA-treated (100 nM for 5 minutes), using Phospho-NMDAR1 (Ser890) Antibody.

IF-F

IF-F

Confocal microscopic image of mouse retina labeled with Phospho-NMDAR1 (Ser890) Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudeocolor stain = DRAQ5® (fluorescent DNA dye).

Background

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor on Ser890/Ser896, and PKA can phosphorylate NR1 on Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment and neuroplasticity (6).

  1. Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
  2. Westphal, R.S. et al. (1999) Science 285, 93-96.
  3. Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
  4. Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-421.
  6. Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.

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