Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

RRM1 Antibody #3388

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk (R) (C) (X) (B) (Dg) Endogenous 90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  X=Xenopus  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RRM1 Antibody detects endogenous levels of total RRM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RRM1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using RRM1 Antibody.

Background

Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).

  1. Mann, G.J. et al. (1988) Cancer Res 48, 5151-6.
  2. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
  3. Ceppi, P. et al. (2006) Ann Oncol 17, 1818-25.
  4. Bepler, G. et al. (2006) J Clin Oncol 24, 4731-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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