Product Pathways - Cytoskeletal Signaling
LPP (8B3A11) Mouse mAb #3389
|3389S||100 µl (10 western blots)||---||In Stock||---|
|3389||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Hamster, Monkey||Endogenous||75||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
LPP (8B3A11) Mouse mAb detects endogenous levels of total LPP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP.
Western blot analysis of extracts from various cell types using LPP (8B3A110) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human small intestine using LPP (8B3A11) Mouse mAb.
Confocal immunofluorescent analysis of COS cells using LPP (8B3A11) Mouse mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1).
LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3).
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