Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

LPP (8B3A11) Mouse mAb #3389

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M Hm Mk Endogenous 75 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LPP (8B3A11) Mouse mAb detects endogenous levels of total LPP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using LPP (8B3A110) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human small intestine using LPP (8B3A11) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of COS cells using LPP (8B3A11) Mouse mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1).LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3).

  1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
  2. Hansen, M.D. and Beckerle, M.C. (2008) Biochem Biophys Res Commun 371, 144-8.
  3. Petit, M.M. et al. (2005) BMC Cell Biol 6, 1.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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