Product Pathways - Tyrosine Kinase/ Adaptors
c-Kit Antibody #3392
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H | 120, 145 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
c-Kit Antibody detects endogenous levels of c-Kit. It does not cross-react with other receptor tyrosine kinase family members.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the residues surrounding Met720 of human c-Kit. The antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of an extract from MO7e cells using c-Kit Antibody.
Western Blotting
Western blot analysis of an extract from Hel cells using c-Kit Antibody.
Background
c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1 and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2 and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
- Martin, F.H. et al. (1990) Cell 63, 203-211.
- Yarden, Y. et al. (1987) EMBO J. 6, 3341-3351.
- Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.
- Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
- Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
- Hirota, S. et al. (1998) Science 279, 577-580.
- Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Application References
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