Cell Signaling Technology

Product Pathways - Neuroscience

NHERF1 (A310) Antibody #3394

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H Endogenous 50 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NHERF1 (A310) Antibody detects endogenous levels of total NHERF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ala310 of human NHERF1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7 and T-47D cells using NHERF1 (A310) Antibody.

IF-IC

IF-IC

Projected confocal z-stack of MCF-7 cells showing staining at the apical membrane when labeled with NHERF1 (A310) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer (4). NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking (6).

  1. Donowitz, M. et al. (2005) J Physiol 567, 3-11.
  2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14.
  3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62.
  4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20.
  5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87.
  6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products