Cell Signaling Technology

Product Pathways - Development

TCF8/ZEB1 (D80D3) Rabbit mAb #3396

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (M) (R) Endogenous 200 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCF8/ZEB1 (D80D3) Rabbit mAb detects endogenous levels of total TCF8/ZEB1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region around residue Asp868 of human TCF8/ZEB1.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected or transfected with a construct expressing human TCF8/ZEB1, using TCF8/ZEB1 (D80D3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, HT1080 and A172 cells using TCF8/ZEB1 (D80D3) Rabbit mAb.

Background

ZEB family proteins are zinc finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1, (ZEB1 and ZEB2 contain two separate Zinc-finger domain and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8).

  1. Vandewalle, C. et al. (2009) Cell Mol Life Sci 66, 773-87.
  2. Aigner, K. et al. (2007) Oncogene 26, 6979-88.
  3. Peinado, H. et al. (2007) Nat Rev Cancer 7, 415-28.
  4. Moreno-Bueno, G. et al. (2008) Oncogene 27, 6958-69.
  5. Krafchak, C.M. et al. (2005) Am J Hum Genet 77, 694-708.
  6. Aldave, A.J. et al. (2007) Am J Med Genet A 143A, 2549-56.
  7. Dastot-Le Moal, F. et al. (2007) Hum Mutat 28, 313-21.
  8. Garavelli, L. and Mainardi, P.C. (2007) Orphanet J Rare Dis 2, 42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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