Myc-Tag (9B11) Mouse mAb (Sepharose Bead Conjugate) #3400
|3400S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|3400||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Transfected Only||Mouse IgG2a|
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-termini of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western blot analysis of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein (lane 1). Immunoprecipitation of COS/CSL lysates with Mouse IgG (Sepharose Bead Conjugate) #3420 (lane 2) or Myc-Tag (9B11) (Sepharose Bead Conjugate) Mouse mAb (lane 3). Blots were probed with Myc Tag (71D10) Rabbit mAb #2278.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Myc-Tag (9B111) Mouse mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Myc-tagged recombinant proteins.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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