Product Pathways - Cytoskeletal Signaling
Myosin Va Antibody #3402
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Myosin Va Antibody detects endogenous levels of total myosin Va heavy chain. Based on sequence homology, the antibody is expected to detect all known myosin Va splice variants.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin Va.
Western blot analysis of extracts from SK-MEL-28 and C2C12 cells using Myosin Va Antibody.
Myosin Va is a molecular motor protein involved in the transport of organelles, vesicles and other cellular cargo along actin filaments (reviewed in 1). The molecule consists of two identical heavy chains, which dimerize via helical domains in a coiled coil structure. The amino-terminal motor domains of the heavy chains contain both the ATPase and the actin-binding activities of myosin Va. The globular tail domains act in a regulatory capacity, binding the myosin Va cargo (2) or inhibiting motor activity by binding the head domains and preventing ATP consumption (3). Mutation of the murine dilute gene, which encodes myosin Va, causes defects in coat pigmentation as well as severe neurological defects (4). In melanocytes, the coiled coil structure of myosin Va is important in regulating the trafficking of melanosomes in conjunction with melanophilin and Rab27a (5). Myosin Va regulates trafficking and exocytosis of secretory granules in neuroendocrine cells (reviewed in 6) as well as RNA transport and distribution (7).
- Desnos, C. et al. (2007) Biol Cell 99, 411-23.
- Wu, X. et al. (1997) J Cell Sci 110 ( Pt 7), 847-59.
- Li, X.D. et al. (2006) J Biol Chem 281, 21789-98.
- Mercer, J.A. et al. (1991) Nature 349, 709-13.
- Hume, A.N. et al. (2006) Mol Biol Cell 17, 4720-35.
- Eichler, T.W. et al. (2006) Biochem Soc Trans 34, 671-4.
- Salerno, V.P. et al. (2008) Cell Motil Cytoskeleton 65, 422-33.
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For Research Use Only. Not For Use In Diagnostic Procedures.