Product Pathways - Companion Products
Mouse IgG (Sepharose Bead Conjugate) #3420
Applications
Immunoprecipitation
Protocols
- 3420:
- Immunoprecipitation
Description
This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Mouse IgG (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Specificity / Sensitivity
Normal mouse IgG is not expected to recognize any specific antigen.
Source / Purification
Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.
IP
Western blot analysis of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein (lane 1). Immunoprecipitation of transfected COS lysates with Mouse IgG (Sepharose Bead Conjugate) (lane 2) or Myc-Tag (9B11) (Sepharose Bead Conjugate) Mouse mAb #3400 (lane 3). Blot was probed with Myc Tag (71D10) Rabbit mAb #2278.
Background
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
