Product Pathways - Complementary Reagents

Mouse IgG (Sepharose Bead Conjugate) #3420

Applications

Immunoprecipitation

Protocols

3420:: Immunoprecipitation

Description

This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Mouse IgG (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

Specificity / Sensitivity

Normal mouse IgG is not expected to recognize any specific antigen.

Source / Purification

Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.

IP

Western blot analysis of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein (lane 1). Immunoprecipitation of transfected COS lysates with Mouse IgG (Sepharose Bead Conjugate) (lane 2) or Myc-Tag (9B11) (Sepharose Bead Conjugate) Mouse mAb #3400 (lane 3). Blot was probed with Myc Tag (71D10) Rabbit mAb #2278.

Background

Control antibodies are used to estimate the non-specific binding of target primary antibodies due to F_c receptor binding or other protein-protein interactions.

Application References

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Companion Products

3900 Rabbit (DA1E) mAb IgG XP^® Isotype Control

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.