Product Pathways - DNA Damage
Phospho-Rad17 (Ser645) Antibody #3421
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk Mi | Endogenous | 80 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Rad17 (Ser645) Antibody detects endogenous levels of hRad17 only when phosphorylated at serine 645. The antibody does not cross-react with Rad17 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser645 of human Rad17. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from JB6 and MvlLu cells, untreated, UV-treated or HU-treated, using Phospho-Rad17 (Ser645) Antibody (upper) or Rad17 Antibody #3422 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-Rad17 (Ser645) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Rad17 (Ser645) Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center) or UV and lambda phosphatase-treated (right) and labeled with Phospho-Rad17 (Ser645) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The human checkpoint protein hRad17 and its fission (Schizosaccharomyces pombe, Rad17) and budding (Saccharomyces cerevisiae, Rad24) yeast orthologues are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR dependent phosphorylation of hRad17 at Ser635 and Ser645. The phosphorylation of hRad17 is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).
- Griffiths, D. J. et al. (1995) EMBO J. 14, 5812-5823.
- Li, L. et al. (1999) Oncogene 18, 1689-1699.
- Bao, S. et al. (1998) Cell Growth Differ. 9, 961-967.
- von Deimling, F. et al. (1999) Hum. Genet. 105, 17-27.
- Bao, S. et al. (2001) Nature 411, 969-973.
- Post, S. et al. (2001) PNAS 98, 13102-13107.
- Wang, X. et al. (2001) Cancer Research 61, 7417-7421.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
