Product Pathways - Complementary Reagents
Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423
| Applications | Reactivity | Isotype |
|---|---|---|
| IP | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3423:
- Immunoprecipitation
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as a control for rabbit IgG monoclonal antibodies.
Source / Purification
IP
Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) (Lanes 1 and 2) or Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Sepharose Bead Conjugate) #3510 (Lanes 3 and 4). The blot was probed using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106.
Description
Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (whole molecule) from Cell Signaling Technology is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Background
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Application References
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Companion Products
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
