Cell Signaling Technology

Product Pathways - DNA Damage

Rad52 Antibody #3425

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk (Hm) Endogenous 40 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rad52 Antibody detects endogenous levels of total Rad52 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Rad52. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, using Rad52 Antibody.

IF-IC

IF-IC

Immunofluorescent analysis of M059J cells, showing localization primarily in the nucleoli, using Rad52 Antibody.

Background

Repair of DNA double-stranded breaks (DSBs) can occur via nonhomologous end joining (NHEJ), homologous recombination (HR), or other DSB repair pathways (1). In yeast, one family of proteins involved in HR is the Rad52 group, which includes Rad52 itself, Rad51, and Rad54 (1). Vertebrate cells lacking Rad51, Rad52, or Rad54 often show enhanced sensitivity to ionizing radiation (IR) or chromosomal abnormatlities (2-4). In response to IR-induced DNA damage, ATM activates the kinase c-Abl, which phosphorylates Rad51 at Tyr54 and Tyr315, and Rad52 at Tyr104, causing translocation of these proteins to sites of DNA strand breaks/subnuclear foci (5-7).

  1. Paques, F. and Haber, J.E. (1999) Microbiol. Mol. Biol. Rev. 63, 349-404.
  2. Bezzubova, O. et al. (1997) Cell 89, 185-193.
  3. Takata, M. et al. (2000) Mol. Cell. Biol. 20, 6476-6482.
  4. Fujimori, A. et al. (2001) EMBO J. 20, 5513-5520.
  5. Chen, G. et al. (1999) J. Biol. Chem. 274, 12748-12752.
  6. Yuan, Z. M. et al. (1998) J. Biol. Chem. 273, 3799-3802.
  7. Kitao, H. and Yuan, Z.M. (2002) J. Biol. Chem. 277, 48944-48948.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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