Cell Signaling Technology

Product Pathways - DNA Damage

Rad50 Antibody #3427

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 153 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rad50 antibody detects endogenous levels of total Rad50 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Rad50. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody.

Background

The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2).

  1. de Jager, M. et al. (2001) Mol. Cell 8, 1129-1135.
  2. Elledge, S.J. (1996) Science 274, 1664-1672.
  3. Lee, J.H. and Lim, D.S. (2006) FEBS J. 273, 1630-1636.
  4. Teng, S.C. et al. (2006) J Mol Histol 37, 293-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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