Product Pathways - Cytoskeletal Signaling
RhoC (D40E4) Rabbit mAb #3430
PhosphoSitePlus® protein, site, and accession data: RhoC
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M Mk (R) | Endogenous | 21 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3430:
- Western Blotting
Specificity / Sensitivity
RhoC (D40E4) Rabbit mAb detects endogenous levels of total RhoC protein. The antibody does not cross-react with RhoA or RhoB.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human RhoC.
Western Blotting
Western blot analysis of extracts from various cell types using RhoC (D40E4) Rabbit mAb.
Western Blotting
Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoC (D40E4) Rabbit mAb.
Background
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).
- DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
Application References
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Companion Products
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
