Cell Signaling Technology

Product Pathways - Development

Id2 (D39E8) Rabbit mAb #3431

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk Endogenous 15 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Id2 (D39E8) Rabbit mAb detects endogenous levels of total Id2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn97 of human Id2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Id2 (D39E8) Rabbit mAb.

Background

Inhibitor of DNA-binding-2 (Id2) is a member of the Id proteins which belong to the helix-loop-helix (HLH) protein family. The Id protein functions by binding to specific transcription factors and preventing their dimerization and DNA binding (1-3). Id2 interacts with a wide variety of transcription factors including E proteins (5), TCS (4), Pax (6) and the tumor suppressor Rb (1). Id2 has been shown to be important in regulating cellular differentiation, proliferation, development and tumorgenesis (7-9). In tumor cells, increased levels of Id2 functionally inactivate Rb, leading to cellular transformation and cancer (10,11). Id2 is therefore a promising therapeutic target for treatment of cancer (12).

  1. Iavarone, A. et al. (1994) Genes Dev 8, 1270-84.
  2. Sun, X.H. et al. (1991) Mol Cell Biol 11, 5603-11.
  3. Roberts, E.C. et al. (2001) Mol Cell Biol 21, 524-33.
  4. Yates, P.R. et al. (1999) EMBO J 18, 968-76.
  5. Engel, I. and Murre, C. (2001) Nat Rev Immunol 1, 193-9.
  6. Roberts, E.C. et al. (2001) Mol Cell Biol 21, 524-33.
  7. Benezra, R. et al. (2001) Oncogene 20, 8334-41.
  8. Lasorella, A. et al. (2001) Oncogene 20, 8326-33.
  9. Zebedee, Z. and Hara, E. (2001) Oncogene 20, 8317-25.
  10. Gabellini, C. et al. (2006) Oncogene 25, 5326-32.
  11. Perk, J. et al. (2005) Nat Rev Cancer 5, 603-14.
  12. Gray, M.J. et al. (2008) Oncogene 27, 7192-200.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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