Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Cytokine Receptor Common beta-Chain Antibody #3432

Applications Reactivity MW (kDa) Source
W H M R Mk Mi 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cytokine Receptor Common beta-Chain Antibody detects endogenous levels of total cytokine receptor common beta-chain protein independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human cytokine receptor common beta-chain. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS, NIH/3T3, HeLa, C6 and MvlLu cells, using Cytokine Receptor Common beta-Chain Antibody.

Background

The common beta-chain (beta-c) of the granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and IL-5 receptors is the major signaling subunit of these receptors, coupling ligand binding to multiple biological activities (1-3). Tyrosine phosphorylation of cytokine receptor common beta-chain is one of the first events in GM-CSF, IL-3 and IL-5 receptor activation and in signaling initiation (4). Serine phosphorylation within the 14-3-3 binding sequence of the common beta-chain is also involved in GM-CSF, IL-3 and IL-5 receptor-specific functions (5,6).

  1. Kitamura, T. et al. (1991) Cell 66, 1165-1174.
  2. Tavernier, J. et al. (1991) Cell 66, 1175-1184.
  3. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 9655-9659.
  4. Muto, A. et al. (1996) J. Exp. Med. 183, 1911-1926.
  5. Stomski, F.C. et al. (1999) Blood 94, 1933-1942.
  6. Guthridge, M.A. et al. (2000) Mol. Cell 6, 99-108.

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