Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Flotillin-2 (C42A3) Rabbit mAb #3436

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 49, 58 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Flotillin-2 (C42A3) Rabbit mAb detects endogenous levels of total flotillin-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe157 of human flotillin-2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Flotillin-2 (C42A3) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells using Flotillin-2 (C42A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Background

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

  1. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
  2. Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
  3. Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
  4. Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
  5. Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
  6. Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
  7. Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
  8. Solomon, S. et al. (2002) Immunobiology 205, 108-19.
  9. Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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