Product Pathways - Wnt / Hedgehog / Notch
Notch1 (5B5) Rat mAb #3447
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PhosphoSitePlus® protein, site, and accession data: Notch 1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R B | Endogenous | 120, 300 | Rat IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Notch1 (5B5) Rat mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1.
Western Blotting
Western blot analysis of extracts from A2780 and A204 cells using Notch1 (5B5) Rat mAb. The transmembrane/intracellular region (NTM) is indicated.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
Application References
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Companion Products
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- 9998 BSA
- 9999 Nonfat Dry Milk
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
