Cell Signaling Technology

Product Pathways - Neuroscience

TDP43 (G400) Antibody #3448

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M R Endogenous 45 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TDP43 (G400) Antibody detects endogenous levels of total TDP43 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly400 of human TDP43. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells and rat brain using TDP43 (G400) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using TDP43 (G400) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

TDP43 (TAR DNA-binding protein 43) is involved in transcriptional regulation and exon splicing (1,2). While normal TDP43 is a nuclear protein, pathological TDP43 is a component of insoluble aggregates in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). In these disorders, TDP43 is abnormally ubiquitinated, phosphorylated and cleaved to generate carboxy-terminal fragments that are sequestered as insoluble aggregates in neuronal nuclei, perikarya, and neurites (3,4). Additionally, TDP43 inhibits the expression of the HIV-1 gene and regulates CFTR gene splicing (1,5).

  1. Ou, S.H. et al. (1995) J Virol 69, 3584-96.
  2. Winton, M.J. et al. (2008) J Biol Chem 283, 13302-9.
  3. Neumann, M. et al. (2006) Science 314, 130-3.
  4. Cairns, N.J. et al. (2007) Am J Pathol 171, 227-40.
  5. Buratti, E. and Baralle, F.E. (2001) J Biol Chem 276, 36337-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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