Product Pathways - Neuroscience
PSD95 (D27E11) XP® Rabbit mAb #3450
PhosphoSitePlus® protein, site, and accession data: PSD-95
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-F | H M R | Endogenous | 95 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PSD95 (D27E11) XP® Rabbit mAb detects endogenous levels of total PSD95 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly99 of human PSD95.
Western Blotting
Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, a SH3 domain and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).
- Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
- Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
- Cai, C. et al. (2006) J. Biol. Chem. 281, 4267-73.
- Yao, W.D. et al. (2004) Neuron 41, 625-38.
- Cline, H. (2005) Curr. Biol. 15, R203-5.
Application References
- Soiza-Reilly, M. and Commons, K.G. (2011) J Comp Neurol 519, 3802-14. Applications: IF-IC (In Cells)
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For Research Use Only. Not For Use In Diagnostic Procedures.
