Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-μ-Opioid Receptor (Ser375) Antibody #3451

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-F M (H) Transfected Only 70 to 90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-µ-Opioid Receptor (Ser375) Antibody detects transfected µ-opioid receptor only when phosphorylated at Ser375 of mouse MOR (or Ser377 of human MOR).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human (homologous to Ser375 of mouse) μ-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from CHO cells, stably transfected with µ-opioid receptor, untreated or DAMGO-treated (1 mM) for various times, using Phospho-µ-Opioid Receptor (Ser375) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from CHO cells, stably transfected with S375A mutant or Wild-type (WT) µ-opioid receptor, untreated or DAMGO-treated (1 mM for 5 minutes), using Phospho-µ-Opioid Receptor (Ser375) Antibody.

IF-F

IF-F

Confocal immunofluorescent analysis of normal mouse cerebellum labeled with Phospho-μ-Opioid Receptor (Ser375) Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides (1). Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase (1) and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades (2,3). Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance (4,5). As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains (6). Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization (7).

  1. Law, P. Y. et al. (2000) Annu. Rev. Pharmacol. Toxicol. 40, 389-430.
  2. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 12402-12406.
  3. Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 23534-23541.
  4. Finn, A.K. and Whistler, J.L. (2001) Neuron 32, 829-839.
  5. Kieffer, B.L. and Evans, C.J. (2002) Cell 108, 587-590.
  6. Yu, Y. et al. (1997) J. Biol. Chem. 272, 28869-28874.
  7. El Kouhen, R. et al. (2001) J. Biol. Chem. 276, 12774-12780.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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