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Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) #3452
| Applications | Reactivity | Source |
|---|---|---|
| F | Rabbit |
Applications Key:
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Source / Purification
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and purified by affinity chromotography. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with a F/P ratio of 2-5.Important Note on Use: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:10 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or etoposide-treated (blue), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9720 compared to Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) (red).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002- and wortmannin-treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2337 compared to Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) (red).
Description
Rabbit IgG was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions, and tested in-house for direct flow cytometric analysis of human and mouse cells. Alexa Fluor® dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugated of the Alexa Fluor® 647 dye produce far-red-fluorescence emission with a peak at 655 nm.
Background
Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type as the test antibody.Important Note on Use: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:10 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.
Application References
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Companion Products
- 2336 Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 3459 Phospho-FLT3 (Tyr591) Antibody (Alexa Fluor® 488 Conjugate)
- 4854 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 4886 Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 9669 Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate)
- 9708 Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate)
- 9719 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 4323 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
