Product Pathways - Tyrosine Kinase/ Adaptors
FLT3 (8F2) Rabbit mAb #3462
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P F | H M | Endogenous | 130 to 160 130 immature form of FLT3. 160 glycosylated mature form of FLT3. | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
FLT3 (8F2) Rabbit mAb detects endogenous levels of FLT3 protein. The antibody does not cross-react with related proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence surrounding Ser740 of human FLT3.
Western Blotting
Western blot analysis of extracts from Baf3/FLT3 transfected cells and SEM leukemia cells, using FLT3 (8F2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded SEM cells, using FLT3 (8F2) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of BaF3 cells, untransfected (red) or transfected with FLT3 (blue), using FLT3 (8F2) Rabbit mAb.
Background
FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
- Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9, 37-48.
- Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30.
- Namikawa, R. et al. (1996) Stem Cells 14, 388-395.
- Beslu, N. et al. (1996) J. Biol. Chem. 271, 20075-20081.
- Zhang, S. and Broxmeyer, H.E. (2000) Biochem. Biophys. Res. Commun. 277, 195-199.
- Zhang, S. et al. (1999) J. Leukoc. Biol. 65, 372-380.
- Mizuki, M. et al. (2000) Blood 96, 3907-3914.
Application References
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Companion Products
- 3463 Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb
- 3464 Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb
- 3466 Phospho-FLT3 (Tyr591) (54H1) Mouse mAb
- 3474 Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb
- 4577 Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb
- 3461 Phospho-FLT3 (Tyr591) Antibody
- 3459 Phospho-FLT3 (Tyr591) Antibody (Alexa Fluor® 488 Conjugate)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
