Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M | 160 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb detects endogenous levels of FLT3 protein only when phosphorylated at tyrosine 589/591. The antibody does not cross-react with other phosphorylated tyrosine kinases.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to the sequences surrounding Tyr589/591 of human FLT3.
Western Blotting
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb specifically recognizes the activated FLT3 but not non-phosphorylated FLT3 protein.
Background
FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
- Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9, 37-48.
- Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30.
- Namikawa, R. et al. (1996) Stem Cells 14, 388-395.
- Beslu, N. et al. (1996) J. Biol. Chem. 271, 20075-20081.
- Zhang, S. and Broxmeyer, H.E. (2000) Biochem. Biophys. Res. Commun. 277, 195-199.
- Zhang, S. et al. (1999) J. Leukoc. Biol. 65, 372-380.
- Mizuki, M. et al. (2000) Blood 96, 3907-3914.
Application References
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Companion Products
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3474 Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb
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- 3462 FLT3 (8F2) Rabbit mAb
- 3463 Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb
- 4577 Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
